The Penguin plans to gain immunity from the law by becoming the police commissioner's son-in-law. He kidnaps Commissioner Gordon's daughter, Barbara, and threatens her into agreeing to marry him. Unknown to him, Barbara Gordon has a secret crime fighting identity, Batgirl. Alfred learns of her secret when he is brought to Penguin's hideout instead of a preacher, and Batman and Robin realize just how valuable their new ally is when they find themselves hopelessly strung up in Penguin's lair.

The Riddler is out to control all of prize fighting in Gotham City. He begins by kidnapping and brainwashing top prizefighters with the aid of the Siren. Then, under the guise of Mushi Nebuchadnezzar, supposed boxing champion of Southwest Asia, he challenges Batman to a fight in the ring. But when it becomes clear that the Riddler has no plans to play fair, it's up to Batgirl to help her crime fighting companions.

Lorelei Circe, aka The Siren, launches a two part plan to become the richest and wickedest woman in Gotham City. First she puts Commissioner Gordon under her stunning spell and orders him to stow away in the Batmobile in order to find out the location of the Batcave as well as Batman & Robins secret identities. Secondly she entrances millionaire playboy Bruce Wayne and implores him to sign over all his wealth and belongings to her.

The Penguin teams up with parasol collector Lola Lasagne. Together, they plan to rig the upcoming Bruce Wayne Handicap to make a fortune from Lola's prize racehorse Parasol. They break into Glu Gluten's Glue Factory to steal a condemned horse, planning to switch horses before the race and make their money by betting on the real Parasol. When Batman, Robin, and Batgirl catch up with them at the glue factory, Penguin sneaks outside and plasters the Batmobile with a sticky glue.

The Penguin steals a priceless folio of famous parasols from the Gotham City Library. He plans to wager the $10,000 earned from its ransom on a rigged horse race. Aided by his partner-in-crime Lola Lasagne, he disguises the favored entry Parasol as the unknown "Bumbershoot," then enters a painted glue factory horse as "Parasol". With everyone betting their money on the fake horse, it looks like the Penguin will make a fortune when the real Parasol wins the race. But he wasn't counting on a last-minute entry of Bruce Wayne's, the horse Waynebow, ridden by none other than Batgirl.

King Tut has been predicting crimes, then secretly making sure they happen in order to gain the police's confidence. He hopes to then send them on a false lead while he commits the crime of the century. After failing an attempt to discover Batman's true identity, he gives his biggest prediction - a jailbreak of several super criminals. While the entire police force is sent to the penitentiary, Tut nabs a collection of priceless Egyptian scrolls from the Gotham City Library. The scrolls contain the key to an ancient statue, rumored to give its possessor control of the universe.

Louie the Lilac has cornered the flower market in Gotham City, hoping to gain control over the entire "flower generation." He kidnaps the organizer of their planned flower-in, Princess Primrose, and brainwashes her with his Stupefying Aromatic Spray. Batman and Robin follow a clue to the villain's lair only to be captured by Louie and fed to his giant man-eating lilacs. With the Caped Crusaders out of the way, Louie heads back to the flower-in to continue his evil plan. Alfred, meanwhile, has been alerted to Batman and Robin's situation, and goes looking for Batgirl to aid in the fight.

Egghead and Olga, Queen of the Bessarovian Cossacks, team up to kidnap Commissioner Gordon. In exchange for his safety, they demand a ten cent tax for every egg eaten in Gotham City. Batman and Robin suspect that Olga may also be after the giant Samovar of Genghis Khan and use it to find a way into her hideout; however, it turns out that the Duo have been expected. While Olga targets Batman as her future husband, her cook prepares a wedding borscht which will contain Commissioner Gordon and Robin.

Egghead and Olga, Queen of the Cossacks, steal two pounds of Radium from the Gotham City Radium Center. Using it in their "Zapping Machine," they attempt to hatch an ancient Neosaurus egg, planning to feed the baby dinosaur a meal of Batman, Robin, and Batgirl. Surprisingly, the machine seems to be working. But when the egg cracks, the baby Neosaurus is more than they can handle.

The Joker plans to become the king of surfing, hoping the fame will give him control over the hearts and minds of Gotham City. He captures top surfer Skip Parker, then uses his "Surfing Experience & Ability Transferometer" to transfer the needed skills and stamina from Skip to himself. When all the other contestants drop out of the upcoming surfing match, Batman steps up to challenge the Joker's supremacy.

Commissioner Gordon travels across the channel to Londinium by request of the President to assist in a spree of baffling burglaries. Also aboard ship are Bruce Wayne, Dick Grayson, their manservant Alfred and Gordon's daughter Barbara. Alfred sets up a make-shift Batcave under a rented mansion near Londinium while Batman & Robin visit lord Ffogg and Lady Peasoup, who run a girl's finishing school that teaches their students a degree in shoplifting.

Lord Ffogg and Lady Peasoup plan to steal a ship-worth of mod clothing material with which they can control the fashion world of Londinium. Ffogg and his men subdue Batman, Peasoup and her girls grab Robin and Lady Prudence uses the paralyzing fog on Batgirl.

Batman, ably assisted as always by Alfred, saves Robin from being crushed by a winch at the Tower of Londinium. He also rescues Batgirl from the dungeon at Ffogg Place. But Robin gets stung by an African Death Bee as Lord Ffogg and Lady Peasoup get ready to steal the crown jewels at the Bloody Tower.

The Catwoman targets the fashion industry by first terrorizing a banquet honoring Batgirl and then attacking a fashion show. During the attack on the fashion show, Catwoman captures Batgirl and takes her back to her hideout where she threatens to kill her with a pattern cutter. She then tells Batman that if he attempts to rescue her that it will leave the visiting Queen Bess of Bellgravia vulnerable for attack. Now the Caped Crusader must find a way to save Batgirl and prevent any harm to Queen Bess.

Egghead and Olga, Queen of the Cossacks, kick off their return with several thefts from the Gotham City Museum. Egghead begins planning a series of heists - intended to provide him with the dowry needed to marry Olga. Swiping 500 pounds of dehydrated caviar goes off without a hitch. But when Batgirl attempts to outfox Egghead, she is instead captured by the villain. It's up to Batman and Robin to find the villains' hideout before Olga's Cossacks make mincemeat out of their crime fighting friend.

The Joker, just released from prison, teams up with Catwoman. They follow the clues of an ancient riddle to retrieve a nightshirt and an antique crib, which together form a map to a hidden cache of gunpowder which they plan to use for their next crime. Batman and Robin, together with Batgirl, follow their trail, little knowing that they are about to walk right into an ambush.

Following a tip from Batgirl, the Dynamic Trio meet up at the Grimalkin Novelty Company, hideout of the Joker and Catwoman. There, they overhear the arch-criminals' plot and secretly trail them to a stash of hidden gunpowder. A careless mistake spoils the villains' evil plan, however, and they are captured and brought before the Gotham City judge. But when the jury seems less than interested in justice, courtroom mayhem ensues.

Louie the Lilac is in town, planning to corner the lilac perfume market in Gotham City. His concoction requires the glands of several animals, so he kidnaps Bruce Wayne and Dick Grayson to make the extractions. With "Batman and Robin" unable to respond, Batgirl attempts a rescue, only to be captured herself. The trio rely on their wits (and the latest bat-invention) to attempt an escape.

Nora Clavicle, a women's rights activist, uses her influence to have Commissioner Gordon, Chief O'Hara, and the whole police force fired and replaced with women. She cuts ties with Batman and Robin, then launches her plan to blow up Gotham City with explosive-packed mice and collect on the insurance. The Terrific Trio look for a way to neutralize her mechanical hordes before it's too late.

The Penguin contaminates all the printed money with Lygerian Sleeping Sickness in order to claim them himself but is dangerously frustrated by Bruce Wayne's countermove.

Shame escapes from prison, and together with Calamity Jan, goes to work on his newest caper. He leaves a cryptic clue for Batman, Robin, and Batgirl describing the first part of his scheme - the robbery of two opera singers. The Terrific Trio decipher his riddle and attempt to stop him, but when Shame breaks out his "fear gas," the three are left quaking in their shoes.

After his previous battle with the Terrific Trio, Shame now has Batgirl as a hostage. He agrees to a swap when he realizes that Batman and Robin also have Calamity Jan's mother, Frontier Fanny. Batgirl informs the Dynamic Duo of a plan for a "great train robbery" that she overheard as a hostage. The three work to figure out Shame's plan and race to stop him. But when they realize they are too late, Batman attempts to lure him out of hiding with an offer of man-to-man combat.

While mining for a rare mineral found only under Wayne Manor, King Tut stumbles upon the Batcave.

The Joker is back in Gotham City, this time with his sights set on worldwide domination. He plants rumors of an invasion from outer space, then sets out to gather the Beryllium metal needed to build an actual flying saucer. Batman picks up on his scheme and sends Alfred to check up on a stash of the metal. But when Alfred is mistaken for a mad scientist by the Joker, he is hauled off to the villain's lair along with Batgirl. The Dynamic Duo are on their way when a bomb planted by one of the Joker's henchmen goes off, leaving them unconscious, and their Batcave cut off from the rest of the world.

Dr. Cassandra has a grand scheme against Gotham City with her invisibility pills merely the beginning of her tricks.

Minerva has been using her "Deepest Secret Extractor," disguised as a piece of equipment at her mineral spa, to discover where her wealthiest customers hide their valuables. With this info in hand, Minerva has been able to pull off a string of robberies with ease. When Batman and Robin's investigation is cut short by the villainess, butler Alfred goes undercover to help foil her criminal operations.